產品中心

當前位置:首頁>產品中心 >ELISA試劑盒 >Rat Elisa Kit

Rat Agrin PicoKine ELISA Kit

Agrin; Agrin N-terminal 110 kDa subunit; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 90 kDa fragment; C90; Agrin C-terminal 22 kDa fragment; C22; Agrn; Agrin

分類:

ELISA試劑盒 - Rat Elisa Kit

說明書:

EK1699

2800元/96T  

cell culture supernates, serum and plasma (heparin, EDTA).

ELISA試劑盒(96T)

現貨

選擇規格:

總價格:

---

選擇數量:

- +

Product Brief

  • 產品概況

    貨號 EK1699
    產品名稱 Rat Agrin PicoKine ELISA Kit
    產品描述 For quantitative detection of rat Agrin in cell culture supernates, serum and plasma (heparin, EDTA).
    免疫原 sf21,A1153-P1959
    交叉反應 There is no detectable cross-reactivity with other relevant proteins.
    敏感性 <10pg/ml
    檢測范圍 31.2pg/ml-2000pg/ml
    基因名稱 Agrn
    基因全名 agrin
    Uniprot ID P25304
    指標別名 Agrin; Agrin N-terminal 110 kDa subunit; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 90 kDa fragment; C90; Agrin C-terminal 22 kDa fragment; C22; Agrn; Agrin
    背景資料 Agrin is a large proteoglycan whose best-characterised role is in the development of the neuromuscular junction during embryogenesis. Agrin is named based on its involvement in the aggregation of acetylcholine receptors during synaptogenesis. In humans, this protein is encoded by the AGRN gene.This protein has nine domains homologous to protease inhibitors.It may also have functions in other tissues and during other stages of development. It is a major proteoglycan component in the glomerular basement membrane and may play a role in the renal filtration and cell-matrix interactions.
    顯色時間 25-30

    試劑盒內容

    SKU 名稱 數量
    Catalog numberDescriptionQuantity
    EK1699-CAP96-well plate precoated with anti- rat Agrin antibody1
    EK1699-STLyophilized recombinant rat Agrin standard10ng/tubex2
    EK1699-DABiotinylated anti- rat Agrin antibody130ul(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex(ABC)130ul(dilution 1:100)
    AR1106-1Sample diluent buffer30ml
    AR1106-2Antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    PLA-SEAAdhesive cover4
    *小貼士:額外的試劑盒部件可以單獨購買。如需購買請和試劑盒一起下單避免額外運費。

    需要用戶自己提供的器材和試劑

    • 1.96孔讀板儀器。
    • 2.自動洗板機.
    • 3.移液器和槍頭。建議使用多通道移液器/排槍。
    • 4.Eppendorf 管。
    • 5.額外洗板緩沖液 (neutral PBS 或 TBS。試劑盒內提供足量。如需額外緩沖液可參考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *樣品準備的相關試劑也不含在試劑盒中。

    批內差/批間差

    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(pg/ml)5721976760223776
    Standard deviation2.3910.7339.13.3614.2750.1
    CV(%)4.2%4.9%5.1%5.6%6.4%7.1%

    Rat Agrin PicoKine ELISA Kit的典型數據示例(取自最近的一批次質檢數據)

    (TMB 孵育條件: 37°C, 25-30)
    Concentration(pg/ml)031.262.512525050010002000
    O.D.0.0790.1560.2110.3360.5650.9911.7412.376
    *典型數據僅供參考。用戶需要自己建立標準曲線。

Instructions

典型操作程序:

1:準備試劑和標準品

2:每孔添加100ul樣品和標準品,37℃反應90分鐘,不洗。

3:每孔添加100ul生物素標記抗體,37℃反應60分鐘。

4:0.01M TBS洗滌3次。

5:每孔添加100ulABC,37℃反應30分鐘。

6:0.01M TBS洗滌5次。

7:TMB37℃避光反應30分鐘以內。

8:加入TMB終止液,讀數。

Related products

暫無相關產品
排列五开奖号码 普通免费单机麻将 体球网手机比分即时比分直播 青海十一选五开奖结果一定牛 江苏麻将全集 单机麻将明星三缺一在线玩 双色球 体彩江苏十一选五规 广西山水麻将游戏下载 澳博瑞特配资 3d开奖号码今晚预测 脱兔电竞比分 pk10ds77