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Mouse SLPI PicoKine ELISA Kit

Antileukoproteinase; ALP; Secretory leukocyte protease inhibitor; Slpi

分類:

ELISA試劑盒 - Mouse Elisa Kit

說明書:

EK1996

2800元/96T  

cell culture supernates, serum and plasma (heparin, EDTA).

ELISA試劑盒(96T)

現貨

選擇規格:

總價格:

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選擇數量:

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Product Brief

  • 產品概況

    貨號 EK1996
    產品名稱 Mouse SLPI PicoKine ELISA Kit
    產品描述 For quantitative detection of mouse SLPI in cell culture supernates, serum and plasma (heparin, EDTA).
    免疫原 NSO, G26-M131
    交叉反應 There is no detectable cross-reactivity with other relevant proteins.
    敏感性 <10pg/ml
    檢測范圍 31.2pg/ml-2000pg/ml
    基因名稱 Slpi
    基因全名 secretory leukocyte peptidase inhibitor
    Uniprot ID P97430
    指標別名 Antileukoproteinase; ALP; Secretory leukocyte protease inhibitor; Slpi
    背景資料 Antileukoproteinase, also known as secretory leukocyte protease inhibitor (SLPI), is an enzyme that in humans is encoded by the SLPI gene. It is mapped to 2; 2 H3. SLPI is a highly cationic single-chain protein with eight intramolecular disulfide bonds. It is found in large quantities in bronchial, cervical, and nasal mucosa, saliva, and seminal fluids. SLPI inhibits human leukocyte elastase, human cathepsin G, human trypsin, neutrophil elastase, and mast cell chymase. X-ray crystallography has shown that SLPI has two homologous domains of 53 and 54 amino acids, one of which exhibits anti-protease activity (C-terminal domain). The other domain (N-terminal domain) is not known to have any function.This gene encodes a secreted inhibitor which protects epithelial tissues from serine proteases. It is found in various secretions including seminal plasma, cervical mucus, and bronchial secretions, and has affinity for trypsin, leukocyte elastase, and cathepsin G. Its inhibitory effect contributes to the immune response by protecting epithelial surfaces from attack by endogenous proteolytic enzymes; the protein is also thought to have broad-spectrum anti-biotic activity.
    顯色時間 15-20

    試劑盒內容

    SKU 名稱 數量
    Catalog numberDescriptionQuantity
    EK1996-CAP96-well plate precoated with anti- mouse SLPI antibody1
    EK1996-STLyophilized recombinant mouse SLPI standard2ng/tube×2
    EK1996-DABiotinylated anti- mouse SLPI antibody130μl(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex (ABC)130μl(dilution 1:100)
    AR1106-1Sample diluent buffer30 ml
    AR1106-2Antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    PLA-SEAAdhesive cover4
    *小貼士:額外的試劑盒部件可以單獨購買。如需購買請和試劑盒一起下單避免額外運費。

    需要用戶自己提供的器材和試劑

    • 1.96孔讀板儀器。
    • 2.自動洗板機.
    • 3.移液器和槍頭。建議使用多通道移液器/排槍。
    • 4.Eppendorf 管。
    • 5.額外洗板緩沖液 (neutral PBS 或 TBS。試劑盒內提供足量。如需額外緩沖液可參考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *樣品準備的相關試劑也不含在試劑盒中。

    批內差/批間差

    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(pg/ml)5921493862237958
    Standard deviation2.4811.3453.473.8413.7560.35
    CV(%)4.2%5.3%5.7%6.2%5.8%6.3%

    Mouse SLPI PicoKine ELISA Kit的典型數據示例(取自最近的一批次質檢數據)

    (TMB 孵育條件: 37°C, 15-20)
    Concentration(pg/ml)031.262.512525050010002000
    O.D.0.0180.1270.1970.4140.7691.3302.0692.463
    *典型數據僅供參考。用戶需要自己建立標準曲線。

Instructions

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