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Mouse FLRT2 PicoKine ELISA Kit

Leucine-rich repeat transmembrane protein FLRT2; Fibronectin-like domain-containing leucine-rich transmembrane protein 2; Flrt2; Kiaa0405

分類:

ELISA試劑盒 - Mouse Elisa Kit

說明書:

EK1972

2800元/96T  

cell culture supernates, serum and plasma (heparin, EDTA).

ELISA試劑盒(96T)

現貨

選擇規格:

總價格:

---

選擇數量:

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Product Brief

  • 產品概況

    貨號 EK1972
    產品名稱 Mouse FLRT2 PicoKine ELISA Kit
    產品描述 For quantitative detection of mouse FLRT2 in cell culture supernates, serum and plasma (heparin, EDTA).
    免疫原 NSO, C36-T660
    交叉反應 There is no detectable cross-reactivity with other relevant proteins.
    敏感性 <10pg/ml
    檢測范圍 31.2pg/ml-2000pg/ml
    基因名稱 Flrt2
    基因全名 fibronectin leucine rich transmembrane protein 2
    Uniprot ID Q8BLU0
    指標別名 Leucine-rich repeat transmembrane protein FLRT2; Fibronectin-like domain-containing leucine-rich transmembrane protein 2; Flrt2; Kiaa0405
    背景資料 Fibronectin leucine-rich repeat transmembrane protein FLRT2 is a protein that in humans is encoded by the FLRT2 gene. It is mapped to 12; 12 E. This gene encodes a member of the fibronectin leucine rich transmembrane (FLRT) family of cell adhesion molecules, which regulate early embryonic vascular and neural development. The encoded type I transmembrane protein has an extracellular region consisting of an N-terminal leucine-rich repeat domain and a type 3 fibronectin domain, followed by a transmembrane domain and a short C-terminal cytoplasmic tail domain. It functions as both a homophilic cell adhesion molecule and a heterophilic chemorepellent through its interaction with members of the uncoordinated-5 receptor family. Proteolytic removal of the extracellular region controls the migration of neurons in the developing cortex. Alternative splicing results in multiple transcript variants.
    顯色時間 20-25

    試劑盒內容

    SKU 名稱 數量
    Catalog numberDescriptionQuantity
    EK1972-CAP96-well plate precoated with anti- mouse FLRT2 antibody1
    EK1972-STLyophilized recombinant mouse FLRT2 standard10ng/tube×2
    EK1972-DABiotinylated anti- mouse FLRT2 antibody130μl(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex (ABC)130μl(dilution 1:100)
    AR1106-1Sample diluent buffer30 ml
    AR1106-2Antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    PLA-SEAAdhesive cover4
    *小貼士:額外的試劑盒部件可以單獨購買。如需購買請和試劑盒一起下單避免額外運費。

    需要用戶自己提供的器材和試劑

    • 1.96孔讀板儀器。
    • 2.自動洗板機.
    • 3.移液器和槍頭。建議使用多通道移液器/排槍。
    • 4.Eppendorf 管。
    • 5.額外洗板緩沖液 (neutral PBS 或 TBS。試劑盒內提供足量。如需額外緩沖液可參考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *樣品準備的相關試劑也不含在試劑盒中。

    批內差/批間差

    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(pg/ml)5223988756265904
    Standard deviation2.189.8039.923.0211.4050.62
    CV(%)4.2%4.1%4.5%5.4%4.3%5.6%

    Mouse FLRT2 PicoKine ELISA Kit的典型數據示例(取自最近的一批次質檢數據)

    (TMB 孵育條件: 37°C, 20-25)
    Concentration(pg/ml)031.262.512525050010002000
    O.D.0.1000.1910.2680.3900.8671.0721.6152.137
    *典型數據僅供參考。用戶需要自己建立標準曲線。

Instructions

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