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Human REG1A PicoKine ELISA Kit

regenerating family member 1 alpha|Lithostathine-1-alpha|Lithostathine-1-alpha; Islet cells regeneration factor; ICRF; Islet of Langerhans regenerating protein; REG; Pancreatic stone protein; PSP; Pancreatic thread protein; PTP; Regenerating islet-derived protein 1-alpha; REG-1-alpha; Regenerating protein I alpha; REG1A; PSPS1

分類:

ELISA試劑盒 - Human Elisa Kit

說明書:

EK1812

2800元/96T  

cell culture supernates, serum and plasma(heparin).

ELISA試劑盒(96T)

現貨

選擇規格:

總價格:

---元*1

選擇數量:

- +

Product Brief

  • 產品概況

    產品名稱Human REG1A PicoKine ELISA Kit
    產品描述For quantitative detection of human REG1A in cell culture supernates, serum and plasma(heparin).
    引用此產品Human REG1A PicoKine ELISA Kit(Boster Biological Technology, Wuhan, China. Catalog #EK1812)
    驗證種屬Human
    免疫原標準品表達系統:NSO, Q23-N166
    交叉反應There is no detectable cross-reactivity with other relevant proteins.
    規格96 wells/kit

    產品性質

    敏感性<10pg/ml
    *敏感性/最低檢測濃度計算方式:敏感性等于20個空白對照孔的平均O.D.值加上2個標準差后對應的濃度。樣品的O.D.讀數只有在高過敏感性指標的時候才具有統計學顯著性。
    檢測范圍31.2pg/ml-2000pg/ml*檢測范圍不代表敏感性。檢測范圍是以常見樣品的指標表達量為參照,在博士德內部質檢過程中使用的標準曲線區間。如果用戶需要,可以加大標準曲線的初始稀釋度來讓試劑盒檢測更低的范圍。更多實驗小貼士參見博士德的技術資料中心
    樣品類型cell culture supernates, serum and plasma(heparin).*對于上述未提及的樣品類型,在一般情況下博士德的ELISA試劑盒可以檢測。由于未檢測的樣品可能出現表達量過低的情況,建議先進行小規模實驗驗證可行性。
    包被抗體A monoclonal antibody from mouse
    檢測抗體a biotinylated detection polyclonal antibody from goat
    一般表達濃度以下文章提供了REG1A的表達量的信息:
      保存條件4℃六個月,-20℃一年。避免不必要的重復溶解、凍結(加冰郵寄)

      試劑盒內容

      SKU名稱數量
      Catalog numberDescriptionQuantity
      EK1812-CAP96-well plate precoated with anti- human REG1A antibody1
      EK1812-STLyophilized recombinant human REG1A standard10ng/tube×2
      EK1812-DABiotinylated anti- human REG1A antibody130ul(dilution 1:100)
      AR1103Avidin-Biotin-Peroxidase Complex (ABC)130ul(dilution 1:100)
      AR1106-1Sample diluent buffer30 ml
      AR1106-2Antibody diluent buffer12ml
      AR1106-3ABC diluent buffer12ml
      AR1104TMB color developing agent10ml
      AR1105TMB stop solution10ml
      PLA-SEAAdhesive cover4
      *小貼士:額外的試劑盒部件可以單獨購買。如需購買請和試劑盒一起下單避免額外運費。

      需要用戶自己提供的器材和試劑

      • 1.96孔讀板儀器。
      • 2.自動洗板機.
      • 3.移液器和槍頭。建議使用多通道移液器/排槍。
      • 4.Eppendorf 管。
      • 5.額外洗板緩沖液 (neutral PBS 或 TBS。試劑盒內提供足量。如需額外緩沖液可參考如下配方)。
        • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
        • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      *樣品準備的相關試劑也不含在試劑盒中。

      指標相關信息 (來源: Uniprot.org)

      小貼士:你可以利用tissue specificity信息來幫助設計陽性和陰性對照。對此有疑問請聯系我們技術服務部門。
      基因名REG1A
      蛋白名Lithostathine-1-alpha
      基因全名regenerating family member 1 alpha
      Uniprot IDREG1A: P05451
      指標別名Lithostathine-1-alpha; Islet cells regeneration factor; ICRF; Islet of Langerhans regenerating protein; REG; Pancreatic stone protein; PSP; Pancreatic thread protein; PTP; Regenerating islet-derived protein 1-alpha; REG-1-alpha; Regenerating protein I alpha; REG1A; PSPS1

      regenerating family member 1 alpha指標簡介

      This gene is a type I subclass member of the Reg gene family. It is mapped to 2p12. The Reg gene family is a multigene family grouped into four subclasses, types I, II, III and IV, based on the primary structures of the encoded proteins. This gene encodes a protein that is secreted by the exocrine pancreas. It is associated with islet cell regeneration and diabetogenesis and may be involved in pancreatic lithogenesis. Reg family members REG1B, REGL, PAP and this gene are tandemly clustered on chromosome 2p12 and may have arisen from the same ancestral gene by gene duplication.

      批內差/批間差

      Intra-Assay PrecisionInter-Assay Precision
      Sample123123
      n161616242424
      Mean(pg/ml)5523490168251998
      Standard deviation2.8615.2151.364.6914.5662.87
      CV(%)5.2%6.5%5.7%6.9%5.8%6.3%

      Human REG1A PicoKine ELISA Kit的典型數據示例(取自最近的一批次質檢數據)

      (TMB 孵育條件: 37°C, 15-20)
      Concentration(pg/ml)031.262.512525050010002000
      O.D.0.1360.1510.1930.2810.4090.6911.3442.338
      *典型數據僅供參考。用戶需要自己建立標準曲線。

    Instructions

    典型操作程序:

    1:準備試劑和標準品

    2:每孔添加100ul樣品和標準品,37℃反應90分鐘,不洗。

    3:每孔添加100ul生物素標記抗體,37℃反應60分鐘。

    4:0.01M TBS洗滌3次。

    5:每孔添加100ulABC,37℃反應30分鐘。

    6:0.01M TBS洗滌5次。

    7:TMB37℃避光反應30分鐘以內。

    8:加入TMB終止液,讀數。

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