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Human Neudesin PicoKine ELISA Kit

Neudesin; Cell immortalization-related protein 2; Neuron-derived neurotrophic factor; Protein GIG47; Secreted protein of unknown function; SPUF protein; NENF; CIR2; SPUF

分類:

ELISA試劑盒 - Human Elisa Kit

說明書:

EK1946

2800元/96T  

cell culture supernates, serum and plasma (heparin, EDTA).

ELISA試劑盒(96T)

現貨

選擇規格:

總價格:

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選擇數量:

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Product Brief

  • 產品概況

    貨號 EK1946
    產品名稱 Human Neudesin PicoKine ELISA Kit
    產品描述 For quantitative detection of human Neudesin in cell culture supernates, serum and plasma (heparin, EDTA).
    免疫原 E.coli, G32-F172
    交叉反應 There is no detectable cross-reactivity with other relevant proteins.
    敏感性 <15pg/ml
    檢測范圍 93.6pg/ml-6000pg/ml
    基因名稱 NENF
    基因全名 neudesin neurotrophic factor
    Uniprot ID Q9UMX5
    指標別名 Neudesin; Cell immortalization-related protein 2; Neuron-derived neurotrophic factor; Protein GIG47; Secreted protein of unknown function; SPUF protein; NENF; CIR2; SPUF
    背景資料 This gene encodes a neurotrophic factor that may play a role in neuron differentiation and development. A pseudogene of this gene is found on chromosome 12. Alternate splicing of this gene results in multiple transcript variants. It is mapped to 1q32.3.
    顯色時間 15-20

    試劑盒內容

    SKU 名稱 數量
    Catalog numberDescriptionQuantity
    EK1946-CAP96-well plate precoated with anti- human Neudesin antibody1
    EK1946-STLyophilized recombinant human Neudesin standard10ng/tube×2
    EK1946-DABiotinylated anti- human Neudesin antibody130μl(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex (ABC)130μl(dilution 1:100)
    AR1106-1Sample diluent buffer30 ml
    AR1106-2Antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    PLA-SEAAdhesive cover4
    *小貼士:額外的試劑盒部件可以單獨購買。如需購買請和試劑盒一起下單避免額外運費。

    需要用戶自己提供的器材和試劑

    • 1.96孔讀板儀器。
    • 2.自動洗板機.
    • 3.移液器和槍頭。建議使用多通道移液器/排槍。
    • 4.Eppendorf 管。
    • 5.額外洗板緩沖液 (neutral PBS 或 TBS。試劑盒內提供足量。如需額外緩沖液可參考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *樣品準備的相關試劑也不含在試劑盒中。

    批內差/批間差

    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(pg/ml)12471520781597392238
    Standard deviation7.6932.18118.457.7942.86140.99
    CV(%)6.2%4.5%5.7%4.9%5.8%6.3%

    Human Neudesin PicoKine ELISA Kit的典型數據示例(取自最近的一批次質檢數據)

    (TMB 孵育條件: 37°C, 15-20)
    Concentration(pg/ml)093.6187375750150030006000
    O.D.0.0580.1190.1810.2570.4820.9101.5222.263
    *典型數據僅供參考。用戶需要自己建立標準曲線。

Instructions

典型操作程序:

1:準備試劑和標準品

2:每孔添加100ul樣品和標準品,37℃反應90分鐘,不洗。

3:每孔添加100ul生物素標記抗體,37℃反應60分鐘。

4:0.01M TBS洗滌3次。

5:每孔添加100ulABC,37℃反應30分鐘。

6:0.01M TBS洗滌5次。

7:TMB37℃避光反應30分鐘以內。

8:加入TMB終止液,讀數。

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