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Human FLT4/VEGFR3 PicoKine ELISA Kit

Vascular endothelial growth factor receptor 3; VEGFR-3; Fms-like tyrosine kinase 4; FLT-4; Tyrosine-protein kinase receptor FLT4; FLT4; VEGFR3

分類:

ELISA試劑盒 - Human Elisa Kit

說明書:

EK0545

2280元/96T  

cell culture supernates, serum and plasma (heparin, EDTA).

ELISA試劑盒(96T)

現貨

選擇規格:

總價格:

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選擇數量:

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Product Brief

  • 產品概況

    貨號 EK0545
    產品名稱 Human FLT4/VEGFR3 PicoKine ELISA Kit
    產品描述 For quantitative detection of human FLT4/VEGFR3 in cell culture supernates, serum and plasma (heparin, EDTA).
    免疫原 NSO, Y25-I776
    交叉反應 There is no detectable cross-reactivity with other relevant proteins.
    敏感性 <50pg/ml
    檢測范圍 156pg/ml-10000pg/ml
    基因名稱 FLT4
    基因全名 fms related tyrosine kinase 4
    Uniprot ID P35916
    指標別名 Vascular endothelial growth factor receptor 3; VEGFR-3; Fms-like tyrosine kinase 4; FLT-4; Tyrosine-protein kinase receptor FLT4; FLT4; VEGFR3
    背景資料 Fms-related tyrosine kinase 4, also known as FLT4, is a protein which in humans is encoded by the FLT4 gene. It is mapped to 5q35.3. This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA.
    顯色時間 15-20

    試劑盒內容

    SKU 名稱 數量
    Catalog numberDescriptionQuantity
    EK0545-CAP96-well plate precoated with anti- human FLT4/VEGFR3 antibody1
    EK0545-STLyophilized recombinant human FLT4/VEGFR3 standard10ng/tube×2
    EK0545-DABiotinylated anti- human FLT4/VEGFR3 antibody130μl(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex (ABC)130μl(dilution 1:100)
    AR1106-1Sample diluent buffer30ml
    AR1106-2Antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    PLA-SEAAdhesive cover4
    *小貼士:額外的試劑盒部件可以單獨購買。如需購買請和試劑盒一起下單避免額外運費。

    需要用戶自己提供的器材和試劑

    • 1.96孔讀板儀器。
    • 2.自動洗板機.
    • 3.移液器和槍頭。建議使用多通道移液器/排槍。
    • 4.Eppendorf 管。
    • 5.額外洗板緩沖液 (neutral PBS 或 TBS。試劑盒內提供足量。如需額外緩沖液可參考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *樣品準備的相關試劑也不含在試劑盒中。

    批內差/批間差

    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(pg/ml)26867029242948543861
    Standard deviation10.9928.81134.5014.9950.39258.69
    CV(%)4.1%4.3%4.6%5.1%5.9%6.7%

    Human FLT4/VEGFR3 PicoKine ELISA Kit的典型數據示例(取自最近的一批次質檢數據)

    (TMB 孵育條件: 37°C, 15-20)
    Concentration(pg/ml)015631262512502500500010000 O.D0.0570.1010.1560.2560.4560.8961.5922.256
    *典型數據僅供參考。用戶需要自己建立標準曲線。

Instructions

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