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Human FCRL5 PicoKine ELISA Kit

Fc receptor like 5|Fc receptor-like protein 5|FcR-like protein 5; FcRL5; BXMAS1; Fc receptor homolog 5; FcRH5; Immune receptor translocation-associated protein 2; CD307e; FCRH5; IRTA2; UNQ503/PRO820

分類:

ELISA試劑盒 - Human Elisa Kit

說明書:

EK1935

2800元/96T  

cell culture supernates, serum and plasma(heparin, EDTA).

ELISA試劑盒(96T)

現貨

選擇規格:

總價格:

---元*1

選擇數量:

- +

Product Brief

  • 產品概況

    產品名稱Human FCRL5 PicoKine ELISA Kit
    產品描述For quantitative detection of human FCRL5 in cell culture supernates, serum and plasma(heparin, EDTA).
    引用此產品Human FCRL5 PicoKine ELISA Kit(Boster Biological Technology, Wuhan, China. Catalog #EK1935)
    驗證種屬Human
    免疫原標準品表達系統:NSO, Q16-G851
    交叉反應There is no detectable cross-reactivity with other relevant proteins.
    規格96 wells/kit

    產品性質

    敏感性<0.1ng/ml
    *敏感性/最低檢測濃度計算方式:敏感性等于20個空白對照孔的平均O.D.值加上2個標準差后對應的濃度。樣品的O.D.讀數只有在高過敏感性指標的時候才具有統計學顯著性。
    檢測范圍0.78ng/ml-50ng/ml*檢測范圍不代表敏感性。檢測范圍是以常見樣品的指標表達量為參照,在博士德內部質檢過程中使用的標準曲線區間。如果用戶需要,可以加大標準曲線的初始稀釋度來讓試劑盒檢測更低的范圍。更多實驗小貼士參見博士德的技術資料中心
    樣品類型cell culture supernates, serum and plasma(heparin, EDTA).*對于上述未提及的樣品類型,在一般情況下博士德的ELISA試劑盒可以檢測。由于未檢測的樣品可能出現表達量過低的情況,建議先進行小規模實驗驗證可行性。
    包被抗體A monoclonal antibody from mouse
    檢測抗體a biotinylated detection polyclonal antibody from goat
    一般表達濃度以下文章提供了FCRL5的表達量的信息:
      保存條件4℃六個月,-20℃一年。避免不必要的重復溶解、凍結(加冰郵寄)

      試劑盒內容

      SKU名稱數量
      Catalog numberDescriptionQuantity
      EK1935-CAP96-well plate precoated with anti- human FCRL5 antibody1
      EK1935-STLyophilized recombinant human FCRL5 standard50ng/tube×2
      EK1935-DABiotinylated anti- human FCRL5 antibody130ul(dilution 1:100)
      AR1103Avidin-Biotin-Peroxidase Complex (ABC)130ul(dilution 1:100)
      AR1106-1Sample diluent buffer30 ml
      AR1106-2Antibody diluent buffer12ml
      AR1106-3ABC diluent buffer12ml
      AR1104TMB color developing agent10ml
      AR1105TMB stop solution10ml
      PLA-SEAAdhesive cover4
      *小貼士:額外的試劑盒部件可以單獨購買。如需購買請和試劑盒一起下單避免額外運費。

      需要用戶自己提供的器材和試劑

      • 1.96孔讀板儀器。
      • 2.自動洗板機.
      • 3.移液器和槍頭。建議使用多通道移液器/排槍。
      • 4.Eppendorf 管。
      • 5.額外洗板緩沖液 (neutral PBS 或 TBS。試劑盒內提供足量。如需額外緩沖液可參考如下配方)。
        • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
        • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      *樣品準備的相關試劑也不含在試劑盒中。

      指標相關信息 (來源: Uniprot.org)

      小貼士:你可以利用tissue specificity信息來幫助設計陽性和陰性對照。對此有疑問請聯系我們技術服務部門。
      基因名FCRL5
      蛋白名Fc receptor-like protein 5
      基因全名Fc receptor like 5
      Uniprot IDFCRL5: Q96RD9
      指標別名FcR-like protein 5; FcRL5; BXMAS1; Fc receptor homolog 5; FcRH5; Immune receptor translocation-associated protein 2; CD307e; FCRH5; IRTA2; UNQ503/PRO820

      Fc receptor like 5指標簡介

      Fc receptor-like protein 5 is a protein that in humans is encoded by the FCRL5 gene. It is mapped to 1q23.1. This gene encodes a member of the immunoglobulin receptor superfamily and the Fc-receptor like family. This gene and several other Fc receptor-like gene members are clustered on the long arm of chromosome 1. The encoded protein is a single-pass type I membrane protein and contains 8 immunoglobulin-like C2-type domains. This gene is implicated in B cell development and lymphomagenesis. Alternatively spliced transcript variants encoding different isoforms have been identified.

      批內差/批間差

      Intra-Assay PrecisionInter-Assay Precision
      Sample123123
      n161616242424
      Mean(pg/ml)10906877211681262689422254
      Standard deviation56.68433.251016.0661.84510.161179.46
      CV(%)5.2%6.3%4.8%4.9%7.4%5.3%

      Human FCRL5 PicoKine ELISA Kit的典型數據示例(取自最近的一批次質檢數據)

      (TMB 孵育條件: 37°C, 20-25)Concentration(ng/ml)
      00.781.563.126.2512.52550
      O.D.0.0120.1090.1410.2580.5931.0071.1441.880
      *典型數據僅供參考。用戶需要自己建立標準曲線。

    Instructions

    典型操作程序:

    1:準備試劑和標準品

    2:每孔添加100ul樣品和標準品,37℃反應90分鐘,不洗。

    3:每孔添加100ul生物素標記抗體,37℃反應60分鐘。

    4:0.01M TBS洗滌3次。

    5:每孔添加100ulABC,37℃反應30分鐘。

    6:0.01M TBS洗滌5次。

    7:TMB37℃避光反應30分鐘以內。

    8:加入TMB終止液,讀數。

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