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Human Cathepsin V PicoKine ELISA Kit

Cathepsin L2; Cathepsin U; Cathepsin V; CTSV; CATL2; CTSL2; CTSU; UNQ268/PRO305

分類:

ELISA試劑盒 - Human Elisa Kit

說明書:

EK1929

2800元/96T  

cell culture supernates, serum and plasma (heparin, EDTA).

ELISA試劑盒(96T)

現貨

選擇規格:

總價格:

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選擇數量:

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Product Brief

  • 產品概況

    貨號 EK1929
    產品名稱 Human Cathepsin V PicoKine ELISA Kit
    產品描述 For quantitative detection of human Cathepsin V in cell culture supernates, serum and plasma (heparin, EDTA).
    免疫原 NSO, V18-V334
    交叉反應 There is no detectable cross-reactivity with other relevant proteins.
    敏感性 <15pg/ml
    檢測范圍 78pg/ml-5000pg/ml
    基因名稱 CTSV
    基因全名 cathepsin V
    Uniprot ID O60911
    指標別名 Cathepsin L2; Cathepsin U; Cathepsin V; CTSV; CATL2; CTSL2; CTSU; UNQ268/PRO305
    背景資料 Cathepsin L2, also known as cathepsin V and encoded by the CTSL2 gene, is a human gene. It is mapped to 9q22.33. The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may play an important role in corneal physiology. This gene is expressed in colorectal and breast carcinomas but not in normal colon, mammary gland, or peritumoral tissues, suggesting a possible role for this gene in tumor processes. Alternatively spliced variants, encoding the same protein, have been identified.
    顯色時間 15-20

    試劑盒內容

    SKU 名稱 數量
    Catalog numberDescriptionQuantity
    EK1929-CAP96-well plate precoated with anti- human Cathepsin V antibody1
    EK1929-STLyophilized recombinant human Cathepsin V standard10ng/tube×2
    EK1929-DABiotinylated anti- human Cathepsin V antibody130μl(dilution 1:100)
    AR1103Avidin-Biotin-Peroxidase Complex (ABC)130μl(dilution 1:100)
    AR1106-1Sample diluent buffer30 ml
    AR1106-2Antibody diluent buffer12ml
    AR1106-3ABC diluent buffer12ml
    AR1104TMB color developing agent10ml
    AR1105TMB stop solution10ml
    PLA-SEAAdhesive cover4
    *小貼士:額外的試劑盒部件可以單獨購買。如需購買請和試劑盒一起下單避免額外運費。

    需要用戶自己提供的器材和試劑

    • 1.96孔讀板儀器。
    • 2.自動洗板機.
    • 3.移液器和槍頭。建議使用多通道移液器/排槍。
    • 4.Eppendorf 管。
    • 5.額外洗板緩沖液 (neutral PBS 或 TBS。試劑盒內提供足量。如需額外緩沖液可參考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *樣品準備的相關試劑也不含在試劑盒中。

    批內差/批間差

    Intra-Assay PrecisionInter-Assay Precision
    Sample123123
    n161616242424
    Mean(pg/ml)10071423461368232464
    Standard deviation5.3044.27105.576.3952.67133.06
    CV(%)5.3%6.2%4.5%4.7%6.4%5.4%

    Human Cathepsin V PicoKine ELISA Kit的典型數據示例(取自最近的一批次質檢數據)

    (TMB 孵育條件: 37°C, 15-20)
    Concentration(pg/ml)078156312625125025005000
    O.D.0.0960.1320.2160.3790.6091.0111.8282.542
    *典型數據僅供參考。用戶需要自己建立標準曲線。

Instructions

典型操作程序:

1:準備試劑和標準品

2:每孔添加100ul樣品和標準品,37℃反應90分鐘,不洗。

3:每孔添加100ul生物素標記抗體,37℃反應60分鐘。

4:0.01M TBS洗滌3次。

5:每孔添加100ulABC,37℃反應30分鐘。

6:0.01M TBS洗滌5次。

7:TMB37℃避光反應30分鐘以內。

8:加入TMB終止液,讀數。

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